Chimeric Antigen Receptor T-Cell Therapy: The Light of Day for Osteosarcoma

T cell receptor recognition of hybrid insulin peptides sure to HLA-DQ8

HLA-DQ8, a genetic threat consider kind I diabetes (T1D), presents hybrid insulin peptides (HIPs) to autoreactive CD4+ T cells. The abundance of spliced peptides binding to HLA-DQ8 and the way they’re subsequently recognised by the autoreactive T cell repertoire is unknown. Right here we report, the HIP, derived from splicing of insulin and islet amyloid polypeptides, generates a most popular peptide-binding motif for HLA-DQ8.

HLA-DQ8-HIP tetramer⁺ T cells from the peripheral blood of a T1D affected person are characterised by repeated TRBV5 utilization, which matches the TCR bias of CD4+ T cells reactive to the HIP peptide remoted from the pancreatic islets of a affected person with T1D. The crystal construction of three TRBV5+ TCR-HLA-DQ8-HIP complexes exhibits that the TRBV5-encoded TCR β-chain types a typical touchdown pad on the HLA-DQ8 molecule.

The N- and C-termini of the HIP is recognised predominantly by the TCR α-chain and TCR β-chain, respectively, in all three TCR ternary complexes. Accordingly, TRBV5 + TCR recognition of HIP peptides would possibly happen by way of a ‘polarised’ mechanism, whereby every chain throughout the αβTCR heterodimer recognises distinct origins of the spliced peptide introduced by HLA-DQ8.

 From a big cohort of consecutive grownup sufferers handled with CAR-T therapies for relapsed or refractory lymphomas from 2016 to 2019, we assessed progression-free survival (PFS), by toxicity improvement (cytokine launch syndrome (CRS), neurotoxicity, or cardiotoxicity]. We additionally assessed the connection of toxicity improvement to goal illness response, and general survival (OS).

Multivariable regression was utilized to judge relationships between customary medical and laboratory measures and illness outcomes. Variations in outcomes, by toxicity standing, have been additionally assessed by way of 30-day landmark evaluation. Moreover, we assessed the results of early anti-CRS toxicity remedy use (at ≤grade 2 toxicity) on most toxicity grade noticed, and long-term illness outcomes (PFS and OS).

Modified-EASIX predicts extreme cytokine launch syndrome and neurotoxicity after Chimeric Antigen Receptor (CAR) T cells

Sufferers who develop CAR T-cells-related extreme cytokine launch syndrome (CRS) and immune-effector-cells-associated neurotoxicity syndrome (ICANS) exhibit hemodynamic instability and endothelial activation. The EASIX (Endothelial-Activation and Stress-Index) rating [lactate dehydrogenase (LDH, U/L) x creatinine (mg/dL) / platelets (10^9 cells/L)] is a marker of endothelial injury that correlates with outcomes in allogeneic hematopoietic cell transplantation.

Elevated LDH and low platelets have been related to extreme CRS and ICANS, as has C-reactive protein (CRP), whereas elevated creatinine is seen solely in a minority of superior extreme CRS instances. We hypothesized that EASIX and two new modified EASIX formulation [simplified-EASIX, which excludes creatinine, and modified-EASIX, which replaces creatinine with CRP (mg/dL)], calculated peri CAR T-cells infusion, could be related to improvement of extreme (grade ≥3) CRS and ICANS.

We included 118 adults, 53 with B-acute lymphoblastic leukemia handled with 1928z CAR T-cells and 65 with diffuse giant B-cell lymphoma handled with axicabtagene-ciloleucel or tisagenlecleucel. The three formulation confirmed related predictive energy for extreme CRS and ICANS. Nevertheless, low platelets and excessive CRP values have been the one variables individually correlated with these toxicities.

CFDA SE Cell Tracer Kit
K1023-1	ApexBio	1 kit	EUR 96
Description: Cell Biology|Cell Proliferation & Toxicity

FluoGreen Tracer, Stains Cell Nuclei and Cytoplasm
MBS555420-01mL	MyBiosource	0.1mL	EUR 435

FluoGreen Tracer, Stains Cell Nuclei and Cytoplasm
MBS555420-5x01mL	MyBiosource	5x0.1mL	EUR 1815

FluoGreen Tracer
SF41000	Neuromics	100 ul	EUR 292.05

CFDA SE cell proliferation and tracer detection kit
ETF0051	EnoGene	2000 assays	EUR 300

CFDA SE cell prolifeRation and tracer detection kit
MBS8579509-2000Assays	MyBiosource	2000Assays	EUR 400

CFDA SE cell prolifeRation and tracer detection kit
MBS8579509-5x2000Assays	MyBiosource	5x2000Assays	EUR 1810

Tau tracer 1
HY-134879	MedChemExpress	Get quote	Ask for price
Description: Tau tracer 1 is a Tau tracer used for imaging Tau protein aggregates. Tau tracer 1 can be used to diagnose neurodegenerative diseases[1].

Tau tracer 2
HY-134880	MedChemExpress	10 mg	EUR 216.45
Description: Tau tracer 2 (Pl-2620) is a Tau tracer used for imaging Tau protein aggregates. Tau tracer 2 can be used to diagnose neurodegenerative diseases[1].

Tau tracer 1
MBS5800094-5mg	MyBiosource	5(mg	EUR 915

Tau tracer 1
MBS5800094-5x5mg	MyBiosource	5x5(mg	EUR 3970

Tau tracer 2
MBS5798041-50mg	MyBiosource	50(mg	EUR 960

Tau tracer 2
MBS5798041-5x50mg	MyBiosource	5x50(mg	EUR 4180

Tau tracer 2
T37051-10mg	TargetMol Chemicals	10mg	Ask for price
Description: Tau tracer 2

Tau tracer 2
T37051-1g	TargetMol Chemicals	1g	Ask for price
Description: Tau tracer 2

Tau tracer 2
T37051-1mg	TargetMol Chemicals	1mg	Ask for price
Description: Tau tracer 2

Tau tracer 2
T37051-50mg	TargetMol Chemicals	50mg	Ask for price
Description: Tau tracer 2

Tau tracer 2
T37051-5mg	TargetMol Chemicals	5mg	Ask for price
Description: Tau tracer 2

Tau tracer 1
T39660-10mg	TargetMol Chemicals	10mg	Ask for price
Description: Tau tracer 1

Tau tracer 1
T39660-1g	TargetMol Chemicals	1g	Ask for price
Description: Tau tracer 1

Tau tracer 1
T39660-1mg	TargetMol Chemicals	1mg	Ask for price
Description: Tau tracer 1

Tau tracer 1
T39660-50mg	TargetMol Chemicals	50mg	Ask for price
Description: Tau tracer 1

Tau tracer 1
T39660-5mg	TargetMol Chemicals	5mg	Ask for price
Description: Tau tracer 1

JAK2 JH2 Tracer
HY-102055	MedChemExpress	50mg	EUR 2705.67
Description: JAK2 JH2 Tracer (Tracer 5) is a fluorescent probe for JAK2 JH2 domain, with a Kd of value 0.2 μM[1][2].

JAK2 JH2 Tracer
MBS3844504-10mg	MyBiosource	10mg	EUR 855

JAK2 JH2 Tracer
MBS3844504-1mg	MyBiosource	1mg	EUR 225

JAK2 JH2 Tracer
MBS3844504-50mg	MyBiosource	50mg	EUR 2450

JAK2 JH2 Tracer
MBS3844504-5mg	MyBiosource	5mg	EUR 525

JAK2 JH2 Tracer
MBS3844504-5x50mg	MyBiosource	5x50mg	EUR 11020

JAK2 JH2 Tracer
MBS5755856-50mg	MyBiosource	50mg	EUR 2840

JAK2 JH2 Tracer
MBS5755856-5x50mg	MyBiosource	5x50mg	EUR 12620

JAK2 JH2 Tracer
T19017-10mg	TargetMol Chemicals	10mg	Ask for price
Description: JAK2 JH2 Tracer

JAK2 JH2 Tracer
T19017-1g	TargetMol Chemicals	1g	Ask for price
Description: JAK2 JH2 Tracer

JAK2 JH2 Tracer
T19017-1mg	TargetMol Chemicals	1mg	Ask for price
Description: JAK2 JH2 Tracer

JAK2 JH2 Tracer
T19017-50mg	TargetMol Chemicals	50mg	Ask for price
Description: JAK2 JH2 Tracer

JAK2 JH2 Tracer
T19017-5mg	TargetMol Chemicals	5mg	Ask for price
Description: JAK2 JH2 Tracer

ROS tracer precursor
HY-126712	MedChemExpress	Get quote	Ask for price
Description: ROS tracer precursor is the precursor of [18F]ROStrace for the synthesis of ROStrace, which can be used for disease diagnosis[1].

ROS tracer precursor
MBS5772039-5mg	MyBiosource	5mg	EUR 915

ROS tracer precursor
MBS5772039-5x5mg	MyBiosource	5x5mg	EUR 3970

ROS tracer precursor
T19550-10mg	TargetMol Chemicals	10mg	Ask for price
Description: ROS tracer precursor

ROS tracer precursor
T19550-1g	TargetMol Chemicals	1g	Ask for price
Description: ROS tracer precursor

ROS tracer precursor
T19550-1mg	TargetMol Chemicals	1mg	Ask for price
Description: ROS tracer precursor

ROS tracer precursor
T19550-50mg	TargetMol Chemicals	50mg	Ask for price
Description: ROS tracer precursor

ROS tracer precursor
T19550-5mg	TargetMol Chemicals	5mg	Ask for price
Description: ROS tracer precursor

CytoFixâ„¢ BCECF, AM *Optimized for long term cellular pH tracking*
21200-1mg	AAT Bioquest	1 mg	EUR 318
Description: Intracellular pH plays an important modulating role in many cellular events, including cell growth, calcium regulation, enzymatic activity, receptor-mediated signal transduction, ion transport, endocytosis, chemotaxis, cell adhesion and other cellular processes.

Long-Term Cell Tracing Reagent CMAC （Blue)
EGY0171	EnoGene	100µg	EUR 92

Long-Term Cell Tracing Reagent CMAC （Blue)
EGY0172	EnoGene	5x100µg	EUR 340

Long-Term Cell Tracing Reagent CMAC (Blue)
MBS8579546-01mg	MyBiosource	0.1mg	EUR 185

Long-Term Cell Tracing Reagent CMAC (Blue)
MBS8579546-5x01mg	MyBiosource	5x0.1mg	EUR 420

Long-Term Cell Tracing Reagent CMFDA（Green）
EGY0181	EnoGene	100µg	EUR 220

Long-Term Cell Tracing Reagent CMFDA（Green）
EGY0182	EnoGene	5x100µg	EUR 500

Long-Term Cell Tracing Reagent CMFDA (Green)
MBS8579547-01mg	MyBiosource	0.1mg	EUR 300

Long-Term Cell Tracing Reagent CMFDA (Green)
MBS8579547-5x01mg	MyBiosource	5x0.1mg	EUR 580

IAA tracer (alkaline phospahatase conjugated)
AS09-517	Agrisera AB	1000 assays	EUR 208

ABA tracer (alkaline phospahatase conjugated)
AS10-726	Agrisera AB	1000 assays	EUR 230

NIR-FLIVO 690 Tracer In vivo Assay
MBS258410-20Tests	MyBiosource	20Tests	EUR 535

NIR-FLIVO 690 Tracer In vivo Assay
MBS258410-5x20Tests	MyBiosource	5x20Tests	EUR 2460

NIR-FLIVO 747 Tracer In vivo Assay
MBS258412-20Tests	MyBiosource	20Tests	EUR 535

NIR-FLIVO 747 Tracer In vivo Assay
MBS258412-5x20Tests	MyBiosource	5x20Tests	EUR 2460

Long-Term Cell Tracing Reagent CDCFDA, SE （Green）
EGY0161	EnoGene	100 µg	EUR 160

Long-Term Cell Tracing Reagent CDCFDA, SE （Green）
EGY0162	EnoGene	5 x 100 µg	EUR 340

Long-Term Cell Tracing Reagent CDCFDA, SE (Green)
MBS8579545-01mg	MyBiosource	0.1mg	EUR 245

Long-Term Cell Tracing Reagent CDCFDA, SE (Green)
MBS8579545-5x01mg	MyBiosource	5x0.1mg	EUR 420

Cis-zeatin tracer (alkaline phospahatase conjugated)
AS16-4092	Agrisera AB	1000 assays	EUR 230

Trans-zeatin tracer (alkaline phospahatase conjugated)
AS16-4090	Agrisera AB	1000 assays	EUR 230

NIR-FLIVO® 690 Tracer In vivo Assay
9112	Immunochemistry	20 Tests	EUR 438

NIR-FLIVO® 747 Tracer In vivo Assay
9114	Immunochemistry	20 Tests	EUR 438

N6-isopentenyladenosine tracer (alkaline phospahatase conjugated)
AS17-4132	Agrisera AB	1000 assays	EUR 208

HRP conjugated mAb anti-Human TNF-alpha (Tracer)
MBS592166-02mL	MyBiosource	0.2mL	EUR 580

HRP conjugated mAb anti-Human TNF-alpha (Tracer)
MBS592166-5x02mL	MyBiosource	5x0.2mL	EUR 2355

CytoSelect™ Cell Transformation Assay (Cell Recovery Compatible), Colorimetric
CBA-135	Cell Biolabs	96 assays	EUR 596

CytoSelect™ Cell Transformation Assay (Cell Recovery Compatible), Colorimetric
CBA-135-5	Cell Biolabs	5 x 96 assays	EUR 2592

CytoSelect™ Cell Transformation Assay (Cell Recovery Compatible), Fluorometric
CBA-140	Cell Biolabs	96 assays	EUR 624

CytoSelect™ Cell Transformation Assay (Cell Recovery Compatible), Fluorometric
CBA-140-5	Cell Biolabs	5 x 96 assays	EUR 2700

Biotin conjugated mAb anti-Human TNF-alpha (Tracer)
MBS592389-01mL	MyBiosource	0.1mL	EUR 580

Biotin conjugated mAb anti-Human TNF-alpha (Tracer)
MBS592389-5x01mL	MyBiosource	5x0.1mL	EUR 2355

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric, Trial Size
CBA-135-T	Cell Biolabs	24 assays	EUR 518.4
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect 96-Well Cell Transformation Assay (Cell Recovery Compatible, Fluorometric), Trial Size
CBA-140-T	Cell Biolabs	24 assays	EUR 547.2
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect™ 96-well Cell Transformation Assay
CBA-130	Cell Biolabs	96 assays	EUR 532

CytoSelect™ 96-well Cell Transformation Assay
CBA-130-5	Cell Biolabs	5 x 96 assays	EUR 2280

CytoSelect 384-well Cell Transformation Assay, Fluorometric
CBA-145	Cell Biolabs	384 assays	EUR 1208.4
Description: Our CytoSelect 384-Well Cell Transformation Assay uses a modified soft agar 3D matrix to support the formation of colonies by neoplastic cells. Quantitation of cell transformation is performed on a fluorescence plate reader.

CytoSelect 384-well Cell Transformation Assay, Fluorometric
CBA-145-5	Cell Biolabs	5 x 384 assays	EUR 4681.2
Description: Our CytoSelect 384-Well Cell Transformation Assay uses a modified soft agar 3D matrix to support the formation of colonies by neoplastic cells. Quantitation of cell transformation is performed on a fluorescence plate reader.

mAb Anti Human PD-L1 clone 12B4, Tracer (Biotin) (Monoclonal)
MBS590085-01mL	MyBiosource	0.1mL	EUR 565

mAb Anti Human PD-L1 clone 12B4, Tracer (Biotin) (Monoclonal)
MBS590085-5x01mL	MyBiosource	5x0.1mL	EUR 2290

mAb Anti-Human PD-1/CD279, 7G12, Tracer (Biotin) (Monoclonal
MBS590084-01mL	MyBiosource	0.1mL	EUR 565

mAb Anti-Human PD-1/CD279, 7G12, Tracer (Biotin) (Monoclonal
MBS590084-5x01mL	MyBiosource	5x0.1mL	EUR 2290

293AD Cell Line
AD-100	Cell Biolabs	1 vial	EUR 308
Description: N/A

293AAV Cell Line
AAV-100	Cell Biolabs	1 vial	EUR 340
Description: N/A

293LTV Cell Line
LTV-100	Cell Biolabs	1 vial	EUR 340
Description: N/A

293RTV Cell Line
RV-100	Cell Biolabs	1 vial	EUR 340

StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)
CBA-325	Cell Biolabs	96 assays	EUR 1027.2
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.

StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)
CBA-325-5	Cell Biolabs	5 x 96 assays	EUR 4033.2
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.

293/GFP Cell Line
AKR-200	Cell Biolabs	1 vial	EUR 388
Description: Fluorescence

T47D/GFP Cell Line
AKR-208	Cell Biolabs	1 vial	EUR 686.4
Description: T47D/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

A549/GFP Cell Line
AKR-209	Cell Biolabs	1 vial	EUR 388
Description: Fluorescence

HeLa/GFP Cell Line
AKR-213	Cell Biolabs	1 vial	EUR 388
Description: Fluorescence

293/Cas9 Cell Line
AKR-5110	Cell Biolabs	1 vial	EUR 460

HeLa/Cas9 Cell Line
AKR-5111	Cell Biolabs	1 vial	EUR 460

NIH3T3/GFP Cell Line
AKR-214	Cell Biolabs	1 vial	EUR 388
Description: Fluorescence

NIH3T3/Cas9 Cell Line
AKR-5104	Cell Biolabs	1 vial	EUR 460

MCF-7/Luc Cell Line
AKR-234	Cell Biolabs	1 vial	EUR 686.4
Description: MCF-7/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line.

SKOV-3/Luc Cell Line
AKR-232	Cell Biolabs	1 vial	EUR 686.4
Description: SKOV-3/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line.

OVCAR-5/RFP Cell Line
AKR-254	Cell Biolabs	1 vial	EUR 686.4
Description: OVCAR-5/RFP Cell Line stably expresses RFP and otherwise exhibits the same characteristics of the parental cell line.

CytoSelect™ 96-Well Cell Transformation Assay (Soft Agar Colony Formation), Trial Size
CBA-130-T	Cell Biolabs	24 assays	EUR 320

Collagen-based Cell Contraction Assay
CBA-201	Cell Biolabs	24 assays	EUR 348

Radius 384-Well Cell Migration Assay
CBA-127	Cell Biolabs	384 assays	EUR 721.2
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 384-Well Cell Migration Assay
CBA-127-5	Cell Biolabs	5 x 384 wells	EUR 2802
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

HIF-1 Alpha Cell Based ELISA Kit
CBA-281	Cell Biolabs	96 assays	EUR 734.4
Description: Cell Biolabs? HIF-1 Cell Based ELISA Kit is an immunoassay developed for rapid detection of HIF-1 Alpha in fixed cells. Cells on a microplate are stimulated for HIF-1 Alpha stabilization, fixed, permeabilized, and then neutralized in the well. HIF-1 Alpha is then detected with an anti-HIF-1 alpha antibody followed by an HRP conjugated secondary antibody. Each kit provides sufficient reagents to perform up to a total of 96 assays and can detect HIF-1 Alpha from human, mouse, or rat.

CytoSelect™ MTT Cell Proliferation Assay
CBA-252	Cell Biolabs	960 assays	EUR 268

Radius™ 24-Well Cell Migration Assay
CBA-125	Cell Biolabs	24 assays	EUR 356
Description: Excepted Quantities

Radius™ 24-Well Cell Migration Assay
CBA-125-5	Cell Biolabs	5 x 24 assays	EUR 1516
Description: Excepted Quantities

Radius™ 96-Well Cell Migration Assay
CBA-126	Cell Biolabs	96 assays	EUR 416
Description: Excepted Quantities

Radius™ 96-Well Cell Migration Assay
CBA-126-5	Cell Biolabs	5 x 96 assays	EUR 1788
Description: Excepted Quantities

Radius™ 48-Well Cell Migration Assay
CBA-5037	Cell Biolabs	48 assays	EUR 372
Description: Excepted Quantities

Radius™ 48-Well Cell Migration Assay
CBA-5037-5	Cell Biolabs	5 x 48 assays	EUR 1592
Description: Excepted Quantities

CytoSelect Cell Proliferation Assay Reagent (Fluorometric)
CBA-250	Cell Biolabs	10 mL	EUR 490.8
Description: Cell Biolabs? CytoSelect Cell Proliferation Assay Reagent (Fluorometric) provides a fluorometric format for measuring and monitoring cell proliferation. Cells can be plated and then treated with compounds or agents that affect proliferation.  Cells are then incubated with the proliferation reagent.  Upon entering metabolically active live cells, the non-fluorescent proliferation reagent is converted into a bright red fluorescent form. An increase in cell proliferation is accompanied by increased fluorescent signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions.  The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues.  This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells. The kit contains sufficient reagents for the evaluation of 960 assays in ten 96-well plates or 192 assays in eight 24-well plates.

CytoSelect™ BrdU Cell Proliferation ELISA Kit
CBA-251	Cell Biolabs	96 assays	EUR 380
Description: Excepted Quantities

CytoSelect™ Cell Viability and Cytotoxicity Assay
CBA-240	Cell Biolabs	96 assays	EUR 256

Platinum-E Retroviral Packaging Cell Line, Ecotropic
RV-101	Cell Biolabs	1 vial	EUR 648

Platinum-GP Retroviral Packaging Cell Line, Pantropic
RV-103	Cell Biolabs	1 vial	EUR 648

CytoSelect 24-well Laminin Cell Invasion, Colorimetric
CBA-110-LN	Cell Biolabs	12 assays	EUR 714
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with Laminin.

Platinum-A Retroviral Packaging Cell Line, Amphotropic
RV-102	Cell Biolabs	1 vial	EUR 648

CytoSelect™ 24-Well Cell Co-Culture System
CBA-160	Cell Biolabs	24 assays	EUR 308

CytoSelect™ 24-Well Cell Co-Culture System
CBA-160-5	Cell Biolabs	5 x 24 assays	EUR 1344

Total Protein - Murine Embryonic Stem Cell Line D3
CBA-305	Cell Biolabs	500 ?g	EUR 414
Description: Isolated from mouse ES-D3 cell line Presented as 500 µg at 1 mg/mL in NP-40 Solubilization Buffer

CytoSelect™ 48-Well Cell Contraction Assay Kit
CBA-5021	Cell Biolabs	48 assays	EUR 484

CytoSelect™ Cell Proliferation Assay Reagent (Colorimetric)
CBA-253	Cell Biolabs	10 mL	EUR 268
Description: Colorimetric

Radius 24-Well Cell Migration Assay, (Laminin Coated)
CBA-125-LN	Cell Biolabs	24 assays	EUR 714
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

StemTAG PCR Primer Set for Stem Cell Characterization
CBA-303	Cell Biolabs	1 kit	EUR 470.4
Description: StemTAG PCR Primer Set for Stem Cell Characterization includes 7 primer pairs: Oct-4, NANOG, AFP, Flk-1, and NCAM, plus GAPDH and beta-actin as controls.

CytoSelect Clonogenic Tumor Cell Isolation Kit (5 preps)
CBA-155	Cell Biolabs	5 preps	EUR 957.6
Description: Many solid tumors contain heterogeneous populations of normal and cancerous cells. Separation of these cell populations is key to an accurate assessment of the true genotypic and phenotypic differences between normal and tumor cells. Our CytoSelect Clonogenic Tumor Cell Isolation Kit uses a proprietary semisolid agar medium to facilitate formation of colonies by cells from solid tumors. Colonies are grown in either a 6-well plate or a 35mm culture dish. These colonies are isolated away from single (i.e. normal) cells by size filtration. The viable cells from these colonies can be easily recovered for further analysis.

Radius 24-Well Cell Migration Assay, (Fibronectin Coated)
CBA-125-FN	Cell Biolabs	24 assays	EUR 714
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

CytoSelect™ 24-well Collagen Cell Invasion, Colorimetric
CBA-110-COL	Cell Biolabs	12 assays	EUR 448
Description: Excepted Quantities

CytoSelect Proliferating Cell Nuclear Antigen (PCNA) ELISA Kit
CBA-254	Cell Biolabs	96 assays	EUR 672
Description: Cell Biolabs? CytoSelect Proliferating Cell Nuclear Antigen (PCNA) ELISA Kit is an enzyme immunoassay developed for the detection and quantitation of PCNA from nuclear and whole cell extracts.  The kit detects PCNA from mouse, rat and human, and has a detection sensitivity limit of 12.5 ng/mLPCNA.  Each kit provides sufficient reagents to perform up to 96 assays including standard curve and unknown samples.

Radius 24-Well Cell Migration Assay, (ECM Array Coated)
CBA-125-ECM	Cell Biolabs	24 wells	EUR 838.8
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

CytoSelect 48-well Cell Adhesion Assay (Laminin, Colorimetric)
CBA-056	Cell Biolabs	48 assays	EUR 512.4
Description: Cell adhesion is a complex process involved in migration/invasion, embryogenesis, wound healing and tissue remodeling. Cells adhere to the extracellular matrix, forming complexes with cytoskeleton components that can affect cell motility, differentiation, proliferation, and survival. Our CytoSelect 48-Well Cell Adhesion Assays provide a fully quantitative method for the evaluation of cell adhesion. The 48-well plate is precoated with Laminin.

CytoSelect 48-well Cell Adhesion Assay (Laminin, Fluorometric)
CBA-057	Cell Biolabs	48 assays	EUR 553.2
Description: Cell adhesion is a complex process involved in migration/invasion, embryogenesis, wound healing and tissue remodeling. Cells adhere to the extracellular matrix, forming complexes with cytoskeleton components that can affect cell motility, differentiation, proliferation, and survival. Our CytoSelect 48-Well Cell Adhesion Assays provide a fully quantitative method for the evaluation of cell adhesion. The 48-well plate is precoated with Laminin.

CytoSelect 24-well Laminin Cell Invasion Assay, Fluorometric
CBA-111-LN	Cell Biolabs	12 assays	EUR 714
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with Laminin.

CytoSelect 96-well Laminin Cell Invasion Assay, Fluorometric
CBA-112-LN	Cell Biolabs	96 assays	EUR 908.4
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Laminin.

CytoSelect 48-well Cell Adhesion Assay (Fibrinogen, Colorimetric)
CBA-058	Cell Biolabs	48 assays	EUR 512.4
Description: Cell adhesion is a complex process involved in migration/invasion, embryogenesis, wound healing and tissue remodeling. Cells adhere to the extracellular matrix, forming complexes with cytoskeleton components that can affect cell motility, differentiation, proliferation, and survival. Our CytoSelect 48-Well Cell Adhesion Assays provide a fully quantitative method for the evaluation of cell adhesion. The 48-well plate is precoated with Fibrinogen.

CytoSelect 48-well Cell Adhesion Assay (Fibrinogen, Fluorometric)
CBA-059	Cell Biolabs	48 assays	EUR 553.2
Description: Cell adhesion is a complex process involved in migration/invasion, embryogenesis, wound healing and tissue remodeling. Cells adhere to the extracellular matrix, forming complexes with cytoskeleton components that can affect cell motility, differentiation, proliferation, and survival. Our CytoSelect 48-Well Cell Adhesion Assays provide a fully quantitative method for the evaluation of cell adhesion. The 48-well plate is precoated with Fibrinogen.

CytoSelect™ 96-well Phagocytosis Assay (Red Blood Cell)
CBA-220	Cell Biolabs	96 assays	EUR 448

Radius™ 24-Well Cell Migration Assay, (Collagen I Coated)
CBA-125-COL	Cell Biolabs	24 assays	EUR 520

Furthermore, solely m-EASIX was a big predictor of illness response. m-EASIX may discriminate sufferers who subsequently developed extreme CRS previous the onset of extreme signs. At day +3, it additionally had excessive discriminatory potential for extreme ICANS (AUC 73%). We suggest m-EASIX as a medical device to probably information individualized administration of sufferers at greater threat for extreme CAR T-cells-related toxicities.